Automated Microinjection

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Autoinjector

Objective Biotechnology’s automated microinjection system enables large-scale microinjection of embryos for a wide range of applications:

  • Automated microinjection in a wide range of species

  • Carry out high-throughput transgenesis or mutagenesis experiments

  • Minimize staff training and hands-on time

  • Increased throughput (hundreds of embryos/hour)

  • Elimination of uninjected embryos

  • Powered by computer vision and machine learning, the automated microinjection robot can be trained to target novel organisms or structures

Automated microinjection system in action

Drosophila melanogaster

Zebrafish

Black Soldier Fly

Aphids

Mosquito embryo

Drosophila pseudoobscurra

Publications:

  1. Andrew D Alegria*, Amey S Joshi*, Jorge Blanco Mendana, Kanav Khosla, Kieran T Smith, Benjamin Auch, Margaret Donovan, John Bischof, Daryl M Gohl, Suhasa B Kodandaramaiah, 'High-throughput genetic manipulation of multi-cellular organisms using a machine-vision guided embryonic microinjection robot' , Genetics, 2024, iyae025, https://doi.org/10.1093/genetics/iyae025, *Equal Contribution

  2. Amey S. Joshi*, Andrew D. Alegria*, Benjamin Auch, Kanav Khosla, Jorge Blanco Mendana, Kunpeng Liu, John Bischof, Daryl M. Gohl, Suhasa B. Kodandaramaiah, 'Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures', IEEE Xplore, Medline/PudMed, IEEE EMBC 2021, *Equal Contribution

  3. Zongqi Guo, Nikolas Zuchowicz, Jessica Bouwmeester, Amey S. Joshi, Amanda L. Neisch, Kieran Smith, Jonathan Daly, Michael L. Etheridge, Erik B. Finger, Suhasa B. Kodandaramaiah, Thomas S. Hays, Mary Hagedorn, John C. Bischof, 'Conduction-Dominated Cryomesh for Organism Vitrification', Advanced Science, 2303317, Nov 2023